Doctoral candidate
Pharmacist
Contact
Institut für Pharmazeutische Technologie und Biopharmazie
Technische Universität Braunschweig
Mendelssohnstraße 1
38106 Braunschweig
E-Mail: hannah.hoelterhoff@tu-braunschweig.de
Room: 035
Telephone: +49 531 391-5660
PVZ / Dep. 4
Room: 360
Telephone: +49 531 391-65655
Field of work
Chip Platform with integrated sensors for dynamic 3D-cellculture models of the intestine and pulmonary tract under hypoxic conditions
As part of the „R2N – Replace und Reduce aus Niedersachsen“ project Micro Replace Systems (2023-2026) will refine the existing Dynamic Micro Tissue Engineering System (DynaMiTES)[1–4], to enable the hypoxic cultivation of cells and organoids. Furthermore, a microfluidic chip will be developed, which does not only allow the precise control of oxygen concentrations but can also be observed under the confocal microscope and will include sensors for a continuous surveillance of culture conditions. These test systems shall be used as animal experiment replacement models in the preclinical development of pharmaceutics.
Funded by Lower Saxony Ministry for Science and Culture and the Volkswagen Stiftung
Press release: Forschung ohne Tierversuche: Was ist möglich?
References
1. Mattern K, Beißner N, Reichl S, Dietzel A (2018) DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 1 - Engineering of microfluidic system and technical simulations. Eur J Pharm Biopharm 126:159–165. doi:10.1016/j.ejpb.2017.04.022
2. Beiβner N, Mattern K, Dietzel A, Reichl S (2018) DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 2 - Ocular DynaMiTES for drug absorption studies of the anterior eye. Eur J Pharm Biopharm 126:166–176. doi:10.1016/j.ejpb.2017.03.021
3. Hinkel S (2020) Parametrische Untersuchung des Einflusses verschiedener Kultivierungsbedingungen auf die Barriereeigenschaften von hCMEC/D3 Zellen unter Verwendung eines dynamischen Zellkulturmodells, Universitätsbibliothek Braunschweig
4. Lorenz T, Kirschke M, Ledwig V, Reichl S, Dietzel A (2021) Microfluidic System for In Vivo-Like Drug Permeation Studies with Dynamic Dilution Profiles. Bioengineering (Basel) 8(5). doi:10.3390/bioengineering8050058