Sponsor: NBank
Contact person: Jennifer Pierick
Summary of the overall project:
Cell-based therapeutic approaches are considered to be one of the most innovative areas of modern medicine and are currently applied primarily in oncology, infection medicine, transplantation and regenerative medicine. The basis of this novel personalized therapy concept is the automated isolation of defined cell populations from blood products. One way of specifically selecting medically relevant cell types is to use small antibody fragments - so-called nanobodies. The aim of the "BioNanoSPION" project is to develop an innovative nanobody production process based on bacterial high-cell density cultivation, which includes a process-integrated purification step using superparamagnetic iron oxide nanoparticles (SPIONs). The corresponding coupling of nanobodies and SPIONs results in conjugates that not only enable simple magnet-based separation of the nanobodies from the culture medium, but also efficient and cost-effective cell isolation by the subsequent end user.
Goals and tasks of iPAT:
Project partner: Institute for Biochemical Engineering (ibvt), IBA Lifesciences GmbH Göttingen
Figure 1: Schematic process of a magnet-based cell isolation
