We will cooperate with several workgroups within consortium in order to make in depth experimental analysis and modelisation to understand the formation and decay of known bacterial signalling molecules in the different projects and to discover new compounds responsible in quorum sensing/quenching issues important in the understanding of phenotypic heterogeneity. The established LC/MS technology for the analysis of signalling molecules will fully be used to answer the questions we rise together with the biologist. In addition we will use the metabolomics approach in some selected experimental setups with our partners. The samples will be profiled in order to achieve a systems map of the basal metabolic profile in time in the various conditions. These studies will use a combination of 'state of the art' analytical tools, including high field cryoNMR spectroscopy, ultrahigh resolution ICR-FT/MS and ultra-performance liquid chromatography (UPLC)-MS together with sophisticated mathematical and computational modeling strategies enabling a metabolic map reconstruction. These methods will be used to generate non-selective profiles of the biological samples generated, thereby providing the widest coverage of the metabolome and to optimize the characterization of the metabolic phenotypes.
References:
- List of publications (P. Schmitt-Kopplin)
Contact details:
PD Dr. Philippe Schmitt-Kopplin
Helmholtz Zentrum München
German Research Center for Environmental Health
Research Unit Analytical BioGeoChemistry (BGC)
Ingolstaedter Landstrasse 1
D-85764 Neuherberg
Tel.: +49-(0)89-3187-3246
Fax.: +49-(0)89-3187-3358
schmitt-kopplin(at)helmholtz-muenchen.de
Homepage Link
Co-workers: Dr. B. Kanawati and Michael Witting (from 01.12.2012)
References:
Schmitt-Kopplin
Anetzberger, C., Reiger, M., Fekete, A., Schell, U., Stambrau, N., Plener, L., Kopka, J., Schmitt-Kopplin, P., Hilbi, H. and Jung, K. (2012) Autoinducers Act as Biological Timers in Vibrio harveyi. PLoS ONE, 7, e48310.
Cataldi, T. R. I., Bianco, G., Fonseca, J. and Schmitt-Kopplin, P. (2012) Perceiving the chemical language of Gram-negative bacteria: listening by high-resolution mass spectrometry. Anal Bioanal Chem.
Lalaouna, D., Fochesato, S., Sanchez, L., Schmitt-Kopplin, P., Haas, D., Heulin, T. and Achouak, W. (2012) Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs. Appl. Environ. Microbiol., 78, 1658-1665.
Schue, M., Fekete, A., Ortet, P., Brutesco, C., Heulin, T., Schmitt-Kopplin, P., Achouak, W. and Santaella, C. (2011) Modulation of metabolism and switching to biofilm prevail over exopolysaccharide production in the response of Rhizobium alamii to cadmium. PLoS ONE, 6, e26771.
Rani, S., Kumar, A., Malik, A. K. and Schmitt-Kopplin, P. (2011) Occurrence of N-Acyl Homoserine Lactones in Extracts of Bacterial Strain of Pseudomonas aeruginosa and in Sputum Sample Evaluated by Gas Chromatography-Mass Spectrometry. American Journal of Analytical Chemistry, 2, 294-302.
Brito-Echeverría, J., Lucio, M., López-López, A., Antón, J., Schmitt-Kopplin, P. and Rosselló-Móra, R. (2011) Response to adverse conditions in two strains of the extremely halophilic species Salinibacter ruber. Extremophiles, 15, 379-389.
Fekete, A., Kuttler, C., Rothballer, M., Hense, B. A., Fischer, D., Buddrus-Schiemann, K., Lucio, M., Müller, J., Schmitt-Kopplin, P. and Hartmann, A. (2010a) Dynamic regulation of N-acyl-homoserine lactone production and degradation in Pseudomonas putida IsoF. FEMS Microbiol. Ecol., 72, 22-34.
Fekete, A., Rothballer, M., Hartmann, A. and Schmitt-Kopplin, P. (2010b) Identification of bacterial autoinducers. In Bacterial SignalingWiley Publishers, Weinheim, Germany, pp. 95-111.
von Rad, U., Klein, I., Dobrev, P. I., Kottova, J., Zazimalova, E., Fekete, A., Hartmann, A., Schmitt-Kopplin, P. and Durner, J. (2008) Response of Arabidopsis thaliana to N-hexanoyl-DL-homoserine-lactone, a bacterial quorum sensing molecule produced in the rhizosphere. Planta, 229, 73-85.
Englmann, M., Fekete, A., Gebefügi, I. and Schmitt-Kopplin, P. (2007a) The dosage of small volumes for chromatographic quantifications using a drop-on-demand dispenser system. Anal Bioanal Chem, 388, 1109-1116.
Pagès, D., Sanchez, L., Conrod, S., Gidrol, X., Fekete, A., Schmitt-Kopplin, P., Heulin, T. and Achouak, W. (2007) Exploration of intraclonal adaptation mechanisms of Pseudomonas brassicacearum facing cadmium toxicity. Environ. Microbiol., 9, 2820-2835.
Englmann, M., Fekete, A., Kuttler, C., Frommberger, M., Li, X., Gebefügi, I., Fekete, J. and Schmitt-Kopplin, P. (2007b) The hydrolysis of unsubstituted N-acylhomoserine lactones to their homoserine metabolites. Analytical approaches using ultra performance liquid chromatography. J Chromatogr A, 1160, 184-193.
Fekete, A., Frommberger, M., Rothballer, M., Li, X., Englmann, M., Fekete, J., Hartmann, A., Eberl, L. and Schmitt-Kopplin, P. (2007) Identification of bacterial N-acylhomoserine lactones (AHLs) with a combination of ultra-performance liquid chromatography (UPLC), ultra-high-resolution mass spectrometry, and in-situ biosensors. Anal Bioanal Chem, 387, 455-467.
Götz, C., Fekete, A., Gebefuegi, I., Forczek, S. T., Fuksová, K., Li, X., Englmann, M., Gryndler, M., Hartmann, A., Matucha, M., Schmitt-Kopplin, P. and Schröder, P. (2007) Uptake, degradation and chiral discrimination of N-acyl-D/L-homoserine lactones by barley (Hordeum vulgare) and yam bean (Pachyrhizus erosus) plants. Anal Bioanal Chem, 389, 1447-1457.
Li, X., Fekete, A., Englmann, M., Götz, C., Rothballer, M., Frommberger, M., Buddrus, K., Fekete, J., Cai, C., Schröder, P., Hartmann, A., Chen, G. and Schmitt-Kopplin, P. (2006) Development and application of a method for the analysis of N-acylhomoserine lactones by solid-phase extraction and ultra high pressure liquid chromatography. J Chromatogr A, 1134, 186-193.
Frommberger, M., Hertkorn, N., Englmann, M., Jakoby, S., Hartmann, A., Kettrup, A. and Schmitt-Kopplin, P. (2005) Analysis of N-acylhomoserine lactones after alkaline hydrolysis and anion-exchange solid-phase extraction by capillary zone electrophoresis-mass spectrometry. Electrophoresis, 26, 1523-1532.