The opportunistic pathogen Legionella pneumophila establishes a distinct replication-permissive vacuole in amoeba and macrophages. For cell-cell communication, the bacteria employ the Lqs (Legionella quorum sensing) system that produces and detects the α-hydroxyketone LAI-1 (Legionella autoinducer-1). The Lqs system includes the autoinducer synthase LqsA, the sensor kinases LqsS and LqsT, and the response regulator LqsR. In this project we analyse the phenotypic heterogeneity of extra- and intracellular L. pneumophila populations in response to small signaling molecules and metabolites.
- List of publications (H. Hilbi)
Contact details:
Prof. Dr. Hubert Hilbi
Ludwig-Maximilians University Munich
Max von Pettenkofer Institute
Pettenkoferstrasse 9a
D-80336 Munich
Tel.: +49-(0)89-2180-72964
Fax: +49-(0)89-2180-99-72964
hilbi@mvp.uni-muenchen.de
Homepage Link
Co-workers: Christian Manske, Ursula Schell, Aline Kessler
References:
Hilbi
Schell, U., Kessler, A. and Hilbi, H. (2014) Phosphorylation signalling through the Legionella quorum sensing histidine kinases LqsS and LqsT converges on the response regulator LqsR. Mol. Microbiol., 92, 1039-1055.
Anetzberger, C., Reiger, M., Fekete, A., Schell, U., Stambrau, N., Plener, L., Kopka, J., Schmitt-Kopplin, P., Hilbi, H. and Jung, K. (2012) Autoinducers Act as Biological Timers in Vibrio harveyi. PLoS ONE, 7, e48310.
Kessler, A., Schell, U., Sahr, T., Tiaden, A., Harrison, C., Buchrieser, C. and Hilbi, H. (2012) The Legionella pneumophila orphan sensor kinase LqsT regulates competence and pathogen-host interactions as a component of the LAI-1 circuit. Environ. Microbiol.
Tiaden, A. and Hilbi, H. (2012) α-Hydroxyketone synthesis and sensing by Legionella and Vibrio. Sensors (Basel), 12, 2899-2919.
Tiaden, A., Spirig, T., Sahr, T., Wälti, M. A., Boucke, K., Buchrieser, C. and Hilbi, H. (2010a) The autoinducer synthase LqsA and putative sensor kinase LqsS regulate phagocyte interactions, extracellular filaments and a genomic island of Legionella pneumophila. Environ. Microbiol., 12, 1243-1259.
Tiaden, A., Spirig, T. and Hilbi, H. (2010b) Bacterial gene regulation by alpha-hydroxyketone signaling. Trends Microbiol., 18, 288-297.
Spirig, T., Tiaden, A., Kiefer, P., Buchrieser, C., Vorholt, J. A. and Hilbi, H. (2008) The Legionella autoinducer synthase LqsA produces an alpha-hydroxyketone signaling molecule. J. Biol. Chem., 283, 18113-18123.
Tiaden, A., Spirig, T., Carranza, P., Brüggemann, H., Riedel, K., Eberl, L., Buchrieser, C. and Hilbi, H. (2008) Synergistic contribution of the Legionella pneumophila lqs genes to pathogen-host interactions. J. Bacteriol., 190, 7532-7547.
Tiaden, A., Spirig, T., Weber, S. S., Brüggemann, H., Bosshard, R., Buchrieser, C. and Hilbi, H. (2007) The Legionella pneumophila response regulator LqsR promotes host cell interactions as an element of the virulence regulatory network controlled by RpoS and LetA. Cell. Microbiol., 9, 2903-2920.