Research overview
I am working on elucidating the biochemical pathways of Withanolide biosynthesis in Nightshade plants. Thereafter, I am particularly interested in studying the evolution of the withanolide biosynthesis genes and their structural diversification. For this, I use long-read sequencing to generate genome sequences. BLAST, synteny analysis, co-expression anlaysis and phylogenetic analysis among others are used to detect gene candidates. 'Data reuse' using publically available genomic and transcriptomie sequences and 'public sharing' is a big component of my work principles.
Qualifications
Publications
Karbstein K., Choudhary N., Xie T., Tomasello S., Wagner N. D., Barke B. H., Paetzold C., Bradican J. P., Preick M., Himmelbach A., Stein N., Papantonis A., Irisarri I., De Vries J., Pucker B., & Hörandl E. (2024). Efficient assembly of plant genomes: A case study with evolutionary implications in Ranunculus (Ranunculaceae). bioRxiv . doi: doi.org/10.1101/2023.08.08.552429
Recinos M. F. M., Winnier S., Lagerhausen K., Ajayi B., Wolff K., Friedhoff R., Dassow C. M., Choudhary N., & Pucker B. (2024). Cacao genome sequence reveals insights into the flavonoid biosynthesis. bioRxiv (Cold Spring Harbor Laboratory). doi: doi.org/10.1101/2024.11.23.624982
Hakim S.E.*, Choudhary N.*, Malhotra K.*, Peng J.*, Arafa A., Bültemeier A., Friedhoff R., Bauer M., Witte C.-P., Herde M., Heretsch P., Pucker B., Franke J. (2024). Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis. bioRxiv 2024.09.27.614867; doi: 10.1101/2024.09.27.614867.
Choudhary N, Pucker B (2024) Conserved amino acid residues and gene expression patterns associated with the substrate preferences of the competing enzymes FLS and DFR. PLOS ONE 19(8): e0305837. doi: 10.1371/journal.pone.0305837.
Rempel A., Choudhary N. and Pucker B. (2023). KIPEs3: Automatic annotation of biosynthesis pathways. PLOS ONE 18(11): e0294342. doi: 10.1371/journal.pone.0294342.
Scholarships and Awards
Conferences, Workshops and Seminars attended
