Supporting Material

Vorlesung Muskelrelaxantien (pdf, 209 KByte)

Special Issue Electrophoresis 2005, "Quantitation and Validation in Electrophoresis"

Quantitation and Validation in Electrophoresis: Definitions and Fundamentals
Hermann Wätzig

• main document
• fig. 1

Precision and variance components in quantitative gel electrophoresis
A.Koller, H.Wätzig

• EXCEL sheet for ANOVA calculation, related to section 3.2, Tables 2 and 3
  
   

Precision from drug stability studies. Collaborative investigation of long-term repeatability and reproducibility of HPLC assay procedures.

Joachim Ermer, Christoph Arth, Phillippe De Raeve, Donald Dill, H.D. Friedel, Heidi Höwer-Fritzen, Gerd Kleinschmidt, Gerhard Köller, Heidi Köppel, Mathias Kramer, Markus Maegerlein, Udo Schepers, Hermann Wätzig

• Precision data from 156 stability studies, discussed in this article
• Spreadsheet used for the calculations
• Template used for the data acquisition
  
   

Out of Specification Test Results from the Statistical Point of View

Heidi Köppel, Berthold Schneider, Hermann Wätzig

• Additional chapter 2.4 (examples)
• Additional third part (discussion about outliers)
• Addendum (e.g. additional Tables)
  
   

Nicht spezifikationskonforme Ergebnisse (Out Of Specification test results) aus statistischer Sicht

Heidi Köppel^a, Berthold Schneider^b, Hermann Wätzig^a
Institut für Pharmazeutische Chemie der TU Braunschweig^a und
Institut für Biometrie der MH Hannover^b

Anhang zum gleichlautenden Artikel in PharmInd in 3 Mitteilungen (im Druck)

courtesy translation

Additional support to the work published in the journal Electrophoresis 2007, 28, 1798 - 1804

• UV - Spectra
  
   

Statistik in der Pharmazeutischen Analytik

Udo Schepers, Hermann Wätzig*

• Teil 3: Statistische Signifikanz und Relevanz: klassische t- und F-Tests oder Äquivalenztests

Strategies in method development to quantify enantiomeric impurities using CE

Sami El Deeb, Phillip Hasemann and Hermann Wätzig*, Electrophoresis 2008, 29, 3552 - 3562

• Additional Tables about screening results

Improving precision in gel electrophoresis by stepwise decreasing variance components -- and considering individual protein properties

Simone Schröder, Asita Brandmüller, Xi Deng, Lothar Jänsch, Aftab Ahmed, Hermann Wätzig*

• EXCEL sheet zu Plakett-Burman design

Trends in der statistischen QC - 1. Teil: Messung

Heidi Köppel, Hermann Wätzig*

• Ergänzungen zum Trendtest nach Wallis und Moore

Trends in der statistischen QC - 3. Teil: Leistungsbewertung

Heidi Köppel, Claudia Cianciulli, Hermann Wätzig*

• Ergänzungen und Erweiterungen

Precision in affinity capillary electrophoresis for drug-protein binding studies

Deia El-Hady, Sascha Kühne, Nagwa El-Maali, Hermann Wätzig*

• Example calculation with EXCEL spreadsheet

Analytical method transfer using equivalence tests with reasonable acceptance criteria and appropriate effort: extension of the ISPE concept

Lukas Kaminski, Udo Schepers, Hermann Wätzig *

• Further comments on equivalence testing
• Example spreadsheet for equivalence interval calculations

Infrared-based temperature measurements in Capillary Electrophoresis

Claudia Cianculli, Hermann Wätzig *

• Additional Figure S1, S2 and Table S1

Quantitative gel electrophoresis: new records in precision by elaborated staining and detection protocols

Xi Deng, Simone Schröder, Sabine Redweik, Hermann Wätzig *

• Excel sheet for ANOVA calculation 2
• Supporting Information
• Explanation to the gel images

• Gel images
   

Fast investigations from biological matrices using CE - test of a blood-brain barrier model

Sascha Kühne, Christopher Untucht, Michael Steinert, Hermann Wätzig *

• Figure S1
• Figure S2
• Figure S3
• Figure S4
• Figure S5
• Figure S6
• Legends of Figures

Statistische Prozesslenkung (SPC)

Wolfgang Bühler, Morteza Farmani, Martin Tegtmeier, Hermann Wätzig *

• Ausführliche Diskussion anhand eines Beispiels (Tablettenmassen)

The challenge to quantify proteins with charge trains due to isoforms or conformers

Xi Deng, Thomas Hahne, Simone Schröder, Sabine Redweik, Dashor Nebiju, Hendrik Schmidt, Ottmar Janssen, Bodo Lachmann, Hermann Wätzig *

• Supporting information

Transfer of Analytical Methods, APV Course 6430, May 10-11, 2012

Katarzyna Lis, Hermann Wätzig *

• Excel-spreadsheet

Analytical instrument qualification in capillary electrophoresis

Claudia Cianciulli, Hermann Wätzig *

• Supplementary information for "Analytical Instrument Qualification in Capillary Electrophoresis"

Precise, fast and flexible determination of protein interactions by affinity capillary electrophoresis: Part 1: Performance

Sabine Redweik, Yuanghong Xu, Hermann Wätzig *

• Supplementary informations: Additional Figures S1, S2, S3

Protein Quantitation using various Modes of High Performance Liquid Chromtography

Sandra Grotefend, Lukas Kaminski, Stefanie Wroblewitz, Sami El Deeb, Nancy Kühn, Stephan Reichl, Markus Limberger, Steven Watt, Hermann Wätzig*

• Protein Quantitation using various Modes of High Performance Liquid Chromtography

The Transfer of Analytical Procedures

Joachim Ermer, Markus Limberger, Katarzyna Lis, Hermann Wätzig*

• Excel-spreadsheet to calculate absolute limits, as well as confidence intervals (CIs) to be compared with acceptance intervals using equivalence tests

Precise, fast and flexible determination of protein interactions by affinity capillary electrophoresis: Part 3: Anions

Yuanghong Xu, Sabine Redweik, Deia Abd El-Hady, Hassan M. Albishiri, Lutz Preu, Hermann Wätzig*

• Supporting information

Investigating the Effects of Sample Pretreatment on Protein Stability Using Size Exclusion Chromatography and High-Resolution Continuum-Source Atomic Absorption Spectrometry

Tobias Rakow , Sami El Deeb , Thomas Hahne , Deia Abd El-Hady, Hassan M. Albishri, Hermann Wätzig*

• Sonication of models proteins
• Recovery rates for different materials
• HR-CS-AAS temperature program
• Freeze-thaw-cycles myoglobin ovalbumin BSA
• Freeze-thaw of model proteins at minus 6 degrees and in liquid nitrogen
• Calculating area of absorbed myoglobin
• Adsorption to filter materials

A Comprehensive Platform to Investigate Protein-Metal Ion Interactions by Affinity Capillary Electrophoresis

Hassan A Alhazmi, Markus Nachbar, Hassan M Albishri, Deia Abd El-Hady, Sabine Redweik, Sami El Deeb, Hermann Wätzig*

• Link to highlights and main findings in presentation (Audioslides)

Data quality in drug discovery - the role of analytical performance in ligand binding assays

Hermann Wätzig, Imke Oltmann-Norden, Franziska Steinicke, Hassan A. Alhazmi, Markus Nachbar, Deia Abd El-Hady, Hassan M Albishri, Knut Baumann, Thomas Exner, Frank M. Böckler, Sami El Deeb.

• Supporting information

Ca2+-complex stability of GAPAGPLIVPY peptide in gas and aqueous phase, investigated by affinity capillary electrophoresis and molecular dynamics simulations and compared to mass spectrometric results

Markus Nachbar, Sami El Deeb, Mona Mozafari, Hassan A Alhazmi, Lutz Preu, Sabine Redweik, Wolf Dieter Lehmann, Hermann Wätzig*

• Movie: The complete disintegration of the proline-rich GAPAGPLIVPY-Ca2+ complex in water within 100 ps (corresponding to Figure 5, calculated by GROMOS 53a6ff; visualization by UCSF Chimera 1.9).

Long term kinetic measurements revealing precision and general performance of surface plasmon resonance biosensors

Franziska Steinicke, Imke Oltmann-Norden, Hermann Wätzig*

• Supporting information

Das Organ im Organ
oder
Das Gehirn an sich

Martin Wätzig
zusammengestellt und nachbearbeitet von Hermann Wätzig

• Titel und Inhalt
• Teil 1
• Teil 2
• Teil 3
• Literatur und Register