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Zangenehzadeh, S., Agocs, E., Schröder, F., Amroun, N., Biedendieck, R., Jahn, D., Günther, A., Zheng, L., Roth, B., Johannes, H.-H. & Kowalsky, W. (2024)
Polarization modulated spectroscopic ellipsometry-based surface plasmon resonance biosensor for E. coli K12 detection.
Sci Rep. 14, 27046
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Zimbelmann, S., Hintmann, M., Baumann, M., Jahn, D., Biedendieck, R. & Emde, B. (2024)
Selective Lethal Impact of Blue Laser Radiation Compared to High-Power LED Irradiation on Naturally Grown Biofouling in the Context of Underwater Biofouling Cleaning.
Procedia CIRP. 124, 696–699
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Wichmann, J., Staar, M., Kubiak, M., Solarczek, J., Mayer, J., Jahn, D., Kampen, I., Schilde, C., Schallmey, A. & Biedendieck, R. (2024)
Correlation of structure and properties of enzyme crystals in consideration of downstream processing and formulation.
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Brune, S.E., Jahn, D., Krull, R. & Biedendieck, R. (2024)
Production, purification, and characterization of recombinant β-lactoglobulin variants.
In "Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes", Kwade, A. and Kampen, I. (Eds.) Springer Nature, 181-200
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Hoppenreijs, L.J.G., Brune, S.E., Biedendieck, R., Krull, R., Boom, R.M. & Keppler, J.K. (2024)
Exploring functionality gain for (recombinant) β-lactoglobulin through production and processing interventions.
Food Bioproc Tech.
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Mingers, T., Mass, V., Barthels, S., Borrero De Acuña, S.M., Biedendieck, R., Cooke, A., Dailey, T., Gerdes, S., Blankenfeld, W., Dailey, H., Warren, M., Jahn, M. & Jahn, D. (2024)
The Alternative Coproporphyrinogen III Oxidase (CgoN) Catalyzes the Oxygen-independent Conversion of Coproporphyrinogen III into Coproporphyrin III.
Front Microbiol. 15, 1378989
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Hintmann, M., Zimbelmann, S., Emde, B., Biedendieck, R. & Jahn, D. (2024)
Antibiotic Effect of High-Power Blue Laser Radiation
Photonics 11, 220
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Brune, S.E., Hoppenreijse, L.J.G., Kühl, T., Lautenbach, V., Walter, J., Peukert, W., Schwarz, K., Imhoff, D., Boom, R.M. Krull, R., Keppler, J.K. & Biedendieck, R. (2023)
Precision fermentation as a route to modify β-lactoglobulin structure through substitution of specific cysteine residues.
Int. Dairy J. 147, 105772
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Weber, J.-N., Minner-Meinen, R., Behnecke, M., Biedendieck, R., Hänsch, V., Hercher, T., van den Hout, L., Knüppel, L., Sivov, S., Schulze, J., Mendel, R.-R., Hänsch, R. & Kaufholdt, D. (2023)
Molybdate transport under the moon light: How the novel AtMOT2 family and GSH-complex formation close gaps in molybdenum homeostasis.
Commun Biol. 6, 801
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Hoppenreijs, L.J.G., Overbeck, A., Brune, S.E., Biedendieck, R., Kwade, A., Krull, R. Boom, R.M. & Keppler, J.K. (2023)
Amyloid-like aggregation of recombinant β-lactoglobulin at pH 3.5 and 7.0: is disulfide bond removal the key to fibrillation?
Int J Biol Macromol. 242, 124855
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Hoppenreijs, L.J.G., Brune, S.E., Biedendieck, R., Krull, R., Boom, R.M. & Keppler, J.K. (2023)
Fibrillation of β-lactoglobulin at pH 2.0: impact of cysteine substitution and disulfide bond reduction
Food Hydrocoll. 141, 108727
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Wichmann, J., Mayer, J., Hintmann, M., Lukat, P., Blankenfeldt, W. & Biedendieck, R. (2023)
Multi-step engineering of a penicillin G acylase for systematic improvement of crystallization efficiency
Cryst Growth Des. 23, 3230–3243
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Zangenehzadeh, S., Agocs, E., Jivani, H., Könemund, L., Neumann, L., Hirschberg, F., Herdan, S., Biedendieck, R., Jahn, D., Roth, B.W., Johannes, H.-H. & Kowalsky, W. (2023)
Bacteria detection in a Kretschmann geometry flow cell at a plasmon-enhanced interface with spectroscopic ellipsometer.
Thin Solid Films., 764, 139583
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Mayer, J., Knuuti, T., Baumgarten, L., Menke, E., Bischoff, L., Bunk, B., & Biedendieck, R. (2022)
Construction and application of a plasmid-based signal peptide library for improved secretion of recombinant proteins with Priestia megaterium.
Microorganisms 10:777
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Könemund, L., Neumann, L., Hirschberg, F., Biedendieck, R., Jahn, D., Johannes, H.-H., & Kowalsky, W. (2022)
Functionalization of an Extended-Gate Field-Effect Transistor (EGFET) for Bacteria Detection.
Sci. Rep., 12, 4397
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Biedendieck, R., Knuuti, T., Moore, S. & Jahn, D. (2021)
The “Beauty in the beast” – the multiple uses of Priestia megaterium in biotechnology
Appl Microbiol Biotechnol 105, 5719-5737
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Kubiak, M., Mayer, J., Kampen, I., Schilde, C. & Biedendieck, R. (2021)
Structure-properties-correlation of cross-linked penicillin G acylase crystals
Crystals 11, 451
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Hofmann, J. D., Biedendieck, R., Michel, A. M., Schomburg, D., Jahn, D. & Neumann-Schaal, M. (2021)
Influence of L-lactate and low glucose concentrations on the metabolism and the toxin formation of Clostridioides difficile,
PLOS ONE, 16, e0244988
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Keppler, J., Heyse, A., Scheidler, E., Uttinger, M., Fitzner, L., Jandt, U., Heyn, T.R., Lautenbach, V., Loch, J., Lohr, J., Kieserling, H., Günther, G., Kempf, E., Grosch, J.-H., Lübbert, C., Lewinski, K., Jahn, D., Peukert, W., Kulozik, U., Drusch, S., Krull, R., Schwarz, K. & Biedendieck, R. (2021)
Towards recombinantly produced milk proteins: Physicochemical and emulsifying properties of engineered whey protein beta-lactoglobulin variants
Food Hydrocoll. 110, 106132
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Kubannek, F., Thiel, S., Bunk, B., Huber, K., Overmann, J., Krewer, U., Biedendieck, R. & Jahn, D (2020)
Performance modelling of the bioelectrochemical glycerol oxidation by a co-culture of Geobacter sulfurreducens and Raoultella electrica
ChemElectroChem. 7:1877-1888
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Godard, T., Zuehlke, D., Richter, G., Wall, M., Rohde, M., Riedel, K., Poblete-Castro, I., Krull, R. & Biedendieck, R. (2020)
Metabolic rearrangements causing elevated proline and polyhydroxybutyrate accumulation during the osmotic adaptation response of Bacillus megaterium
Front. Bioeng. Biotechnol. 8:47
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Engel, C. E. A., Vorländer, D., Biedendieck, R., Krull, R. & Dohnt, K. (2020)
Quantification of microaerobic growth of Geobacter sulfurreducens.
PLOS One in press
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Thiel, S., Bunk, B., Spröer, C., Overmann, J., Jahn, D. & Biedendieck, R. (2019)
Complete genome sequence of Raoultella electrica 1GB (DSM 102253T), isolated from anodic biofilms of a glucose-fed microbial fuel cell.
Microbiol Resour Announc 8. pii: e00800-19
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Kleinfeldt, L., Gädke, J., Biedendieck, R., Krull, R. & Garnweitner, G. (2019)
Spray-dried hierarchical aggregates of iron oxide nanoparticles and their functionalization for downstream processing in biotechnology.
ACS Omega 4:16300-16308
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Mayer, J., Pippel, J., Günther, G., Müller, C., Lauermann, A., Knuuti, T., Blankenfeldt, W., Jahn, D. & Biedendieck, R. (2019)
Crystal structures and protein engineering of three different penicillin G acylases from Gram-positive bacteria with different thermostability.
Appl Microbiol Biotechnol. 103:7537-7552
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Münch, K., Münch, R., Biedendieck, R., Jahn, D. & Müller, J. (2019)
Evolutionary model for the unequal segregation of high copy plasmids.
PLOS Comp Biol. 15:e1006724
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Hofmann, J. D., Otto, A., Berges, M., Biedendieck, R., Michel, A.-M., Becher, D., Jahn, D. & Neumann-Schaal, M. (2018)
Metabolic reprogramming of Clostridioides difficile during the stationary phase with the induction of toxin production.
Front Microbiol. 9:1970
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Moore, S.J., MacDonald, J.T., Wienecke, S., Ishwarbhai, A., Tsipa, A., Aw, R., Kylilis, N., Bell, D.J., McClymont, D.W., Jensen, K., Polizzi, K.M., Biedendieck, R. & Freemont, P.S. (2018)
Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria
Proc Natl Acad Sci USA. 115:E4340-E4349
PUBMED: 28917535
Gädke, J., Thies, J.W., Kleinfeldt, L., Schulze, T., Biedendieck, R., Rustenbeck, I., Garnweitner, G., Krull, R. & Dietzel, A. (2018)
Selective manipulation of superparamagnetic nanoparticles for product purification and microfluidic diagnostics.
Eur J Pharm Biopharm. 126:67-74
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Lakowitz, A., Godard, T., Biedendieck, R. & Krull, R. (2018)
Mini review: Recombinant production of tailored bio-pharmaceuticals in different Bacillus strains and future perspectives
Eur J Pharm Biopharm. 126:27-39
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Gädke, J., Thies, J.-W., Kleinfeldt, L., Kalinin, A., Starke, G., Lakowitz, A., Biedendieck, R., Garnweitner, G., Dietzel, A. & Krull, R. (2017)
Integrated in situ-purification of recombinant proteins from Bacillus megaterium cultivations using SPION in stirred tank reactors.
Biochem Eng J. 126: 58-67
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Blaženović, I., Kind, T., Torbašinović, H., Obrenović, S., Mehta, S.S., Tsugawa, H., Wermuth, T., Schauer, N., Jahn, M., Biedendieck, R., Jahn, D. & Fiehn, O. (2017)
Comprehensive comparison of in silico MS/MS fragmentation tools of the CASMI contest: database boosting is needed to achieve 93% accuracy
J Cheminform. 9:32
PUBMED: 28115011
Lakowitz, A., Krull, R. & Biedendieck, R. (2017)
Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains.
Microb Cell Fact. 16: 14
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Gädke, J., Kleinfeldt, L., Schubert, C., Rohde, M., Biedendieck, R., Garnweitner, G. & Krull, R. (2017)
In situ affinity purification of His-tagged protein A from Bacillus megaterium cultivation using recyclable superparamagnetic iron oxide nanoparticles.
J Biotechnol. 242: 55-63
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Ferrer, A., Bunk, B., Spröer, C., Biedendieck, R., Valdés, N., Jahn, M., Jahn, D., Orellana, O. & Levican, G. (2016)
Complete genome sequence of the bioleaching bacterium Leptospirillum sp. group II strain CF-1.
J Biotechnol. 222: 21-22.
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A Bacillus megaterium system for the production of recombinant proteins and protein complexes - in "Advances in Experimental Medicine and Biology / Advanced Technologies for Protein Complex Production" (Vega, C., Ed.)
Springer, Berlin, Heidelberg pp. 97-113
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Gerber, A., Kleser, M., Biedendieck, R., Bernhardt, R. & Hannemann, F. (2015)
Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.
Microb Cell Fact. 14:107
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Münch, K., Müller, J., Wienecke, S., Bergmann, S., Heyber, S., Biedendieck, R., Münch, R. & Jahn, D. (2015)
Polar fixation of plasmids during recombinant protein production in Bacillus megaterium results in population heterogeneity.
Appl Environ Microbiol. 81:5976-5986
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Finger, C., Gamer, M., Klunkelfuß, S., Bunk, B. & Biedendieck, R. (2015)
Impact of rare codons and the functional coproduction of rate-limiting tRNAs on recombinant protein production in Bacillus megaterium
Appl Microbiol Biotechnol. 99:8999-9010.
Masthoff, I., Gädke, J., Krull, R., Biedendieck, R. & Garnweitner (2014)
Funktionalisierte Nanopartikel - Biokompatible Eisenoxid-Nanopartikel zur in situ-Magnetseparation von Bioprodukten
CITplus 10:46-48.
Schulz, A., Fräger, M., Holtkamp, L., Ronnenberg, J. & Biedendieck, R. (2014)
Bacillus megaterium - ein Produktionssystem für rekombinante Proteine.
BIOspektrum 06:650-651.
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Moore, S.J., Mayer, M.J., Biedendieck, R., Deery, E. & Warren, M. (2014)
Towards a cell factory for vitamin B12 production in Bacillus megaterium: Bypassing of the cobalamin riboswitch control elements.
N Biotechnol. 31:553-561.
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Moore, S.J., Lawrence, A.D., Biedendieck, R., Deery, E., Frank, S., Howard, M.J., Rigby, S.E.J. & Warren, M.J. (2013)
Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12).
Proc Natl Acad Sci USA 110: 14906-149011.
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Collins, H.F., Biedendieck, R., Leech, H.K., Gray, M., Escalante-Semerena, J.C., McLean, K.J., Munro, A.W., Rigby, S.E., Warren, M.J. & Lawrence, A.D. (2013)
Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species.
PLoS One 8: e55708.
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Buschke, N., Becker, J., Schäfer, R., Kiefer, P., Biedendieck, R. & Wittmann, C. (2013)
Systems metabolic engineering of xylose utilizing Corynebacterium glutamicum for production of 1,5-diaminopentane.
Biotechnol J. 8: 557-570.
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Korneli, C., David, F., Biedendieck, R., Jahn, D. & Wittmann, C. (2013)
Getting the big beast to work--systems biotechnology of Bacillus megaterium for novel high-value proteins
J Biotechnol. 163: 87-96.
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Moore, S.J., Biedendieck, R., Lawrence, A.D., Deery, E., Howard, M.J., Rigby, S.E.J. & Warren, M.J. (2013)
Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway.
J Biol Chem. 288: 297-305.
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Korneli, C., Biedendieck, R., David, F., Jahn, D. & Wittmann, C. (2013)
High yield production of extracellular recombinant levansucrase by Bacillus megaterium.
Appl Microbiol Biotechnol. 97: 3343-3353.
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Klein, J., Leupold, S., Biegler, I., Biedendieck, R., Münch, R. & Jahn, D. (2012)
TLM-Tracker: Software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies.
Bioinformatics 28: 2276-2277.
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Borgmeier, C., Biedendieck, R., Hoffmann, K., Jahn, D. & Meinhardt, F. (2011)
Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression.
Appl Microbiol Biotechnol. 92: 583-596.
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Eppinger, M., Bunk, B., Johns, M.A., Edirisinghe, J.N., Kutumbaka, K.K., Koenig, S.S.K., Creasy, H.H., Rosovitz, M.J., Riley, D.R., Daugherty, S., Martin, M., Elbourne, L.D.H., Paulsen, I., Biedendieck, R., Braun, C., Grayburn, Dhingra, S., Lukyanchuk, V., Ball, B., ul-Qamar, R., Seibel, J., Bremer, E., Jahn, D., Ravel, J. & Vary, P.S. (2011)
Genome sequences of the biotechnologically important B. megaterium strains QM B1551 and DSM319.
J Bacteriol. 193: 4199-4213.
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Strube, C.P., Homann, A., Gamer, M., Jahn, D., Seibel, J. & Heinz, D.W. (2011)
Polysaccharide synthesis of the levansucrase SACB from Bacillus megaterium is controlled by distinct surface motifs.
J Biol Chem. 286: 17593-17600.
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Biedendieck, R., Borgmeier, C., Bunk, B., Stammen, S., Scherling, C., Meinhardt, F., Wittmann, C. & Jahn, D. (2011)
Systems biology of recombinant protein production using Bacillus megaterium.
Methods Enzymol. 500: 165-195.
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Stammen, S., Schuller, F., Dietrich, S., Gamer, M., Biedendieck, R. & Jahn, D. (2010)
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium.
Appl Microbiol Biotechnol. 88: 529-539.
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Stammen, S., Müller, B.K., Korneli, C., Biedendieck, R., Gamer, M., Franco-Lara, E. & Jahn, D. (2010)
High yield intra- and extracellular protein production using Bacillus megaterium.
Appl Environ Microbiol. 76: 4037-4046.
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Bunk, B., Schulz, A., Stammen, S., Münch, R., Warren, M. J., Rohde, M., Jahn, D. & Biedendieck, R. (2010)
A short story about a big magic bug.
Bioeng Bugs 1: 1-7.
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Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J.-H., Deery, E., Leech, H., Warren, M. J. & Jahn, D. (2010)
Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium.
Microb Biotechnol. 3: 24-37.
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Bacillus megaterium and other Bacilli, Industrial Applications
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Möbius, K., Arias-Cartin, R., Breckau, D., Haennig, A.-L., Riedmann, K., Biedendieck, R., Schroeder, S., Becher, D., Magalon, A., Moser, J., Jahn, M. & Jahn, D. (2010)
Heme biosynthesis is coupled to electron transport chains for energy generation
Proc. Natl. Acad. Sci. USA 107:10436-10441.
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Biedendieck, R., Bunk, B., Fürch, T., Franco-Lara, E., Jahn, M. & Jahn, D. (2010)
Systems Biology of Recombinant Protein Production in Bacillus megaterium - in "Advances in Biochemical Engineering / Biotechnology"
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Springer, Berlin, Heidelberg 120: 133-161.
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Schroeder, S., Lawrence, A.D., Biedendieck, R., Rose, R.S., Deery, E., Graham, R.M., McLean, K.J., Munro, A.W., Rigby, S.E. & Warren, M.J. (2009)
Demonstration that CobG, the monooxygenase associated with the ring contraction process of the aerobic cobalamin (vitamin B12) biosynthetic pathway, contains an Fe-S center and a mononuclear non-heme iron center.
J Biol Chem 284: 4796-4805.
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Beine, R., Valente, A.R., Biedendieck, R., Jahn, D. & Seibel, J. (2009)
Directed optimization of biocatalytic transglycosylation processes by the integration of genetic algorithms and fermentative approaches into a kinetic model.
Proc Biochem. 44: 1103-1114.
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Gamer, M., Froede, D., Biedendieck, R., Stammen, S., Jahn, D. (2009)
A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium.
Appl Microbiol Biotechnol. 82: 1195-203.
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Biedendieck, R., Gamer, M., Jaensch, L., Meyer, S., Rohde, M., Deckwer, W.-D. & Jahn, D. (2007)
A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.
J Biotechnol. 132: 426-430.
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Homann, A., Biedendieck, R., Goetze, S., Jahn, D. & Seibel, J. (2007)
Insights into polymer versus oligosaccharide synthesis - Mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium.
Biochem J. 407:189-198.
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Bäumchen, C., Roth, A. H. F. J., Biedendieck, R., Malten, M., Follmann, M., Sahm, H., Bringer-Meyer, S. & Jahn, D. (2007)
D-Mannitol production by resting state whole cell biotransformation of D-fructose by heterologous mannitol and formate dehydrogenase genes expression in Bacillus megaterium.
Biotechnol J. 2:1408-1416
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Vary, P. S., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W.-D. & Jahn, D. (2007)
Bacillus megaterium - from simple soil bacterium to industrial protein production host.
Appl Microbiol Biotechnol. 76:957-967. Review.
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Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D. & Duebel, S. (2007)
Production of recombinant antibody fragments in Bacillus megaterium
Microb Cell Fact. 6:2.
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Biedendieck, R., Beine, R., Gamer, M., Jordan, E., Buchholz, K., Seibel, J., Dijkhuizen, L., Malten, M. & Jahn, D. (2007)
Export, purification and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium.
Appl Microbiol Biotechnol. 74:1062-1073.
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Biedendieck, R., Yang, Y., Deckwer, W.-D., Malten, M. & Jahn, D. (2007)
Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium.
Biotechnol Bioeng. 96:525-537.
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Yang, Y., Biedendieck, R., Wang, W., Gamer, M., Malten, M., Jahn, D. & Deckwer, W.-D. (2006)
High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium.
Microb Cell Fact. 5:36.
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Malten, M., Biedendieck, R., Gamer, M., Drews, A.C., Stammen, S., Buchholz, K., Dijkhuizen, L. & Jahn D (2006)
A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium.
Appl Environ Microbiol. 72:1677-1679.
Hollmann, R., Malten, M., Biedendieck, R., Yang, Y., Wang, W., Jahn, D. & Deckwer, W.-D. (2006)
Bacillus megaterium als Produktionssystem für rekombinante Proteine
Chemie Ingenieur Technik 78:289-294.